Venable, Baetjer, Howard & Civiletti, LLP Fei-Fei Chao
Chimeric proteins with a cellulose binding domain
Chimeric proteins containing a cellulose binding domain (CBD) situated between two independent desired proteins of different lengths can be contemplated for use in a wide range of applications. In addition to its affinity to cellulose due to the presence of the CBD, various properties can be adopted to the chimeric protein by means of introducing the desired proteins upstream and downstream of the CBD. For example, a chimeric protein is produced in Escherichia coli in large quantities by joining a thioredoxin, a CBD and a short peptide composed of 3 amino acid (i.e., Arginine-Glutamate-Asparate). This chimeric protein can be further purified in simple steps and added to cell culture to enhance cell attachment to a surface made up of cellulose.
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C12N C12Y C12N C07K
inventional inventional inventional additional
Tzong-Hsiung Nien-Tai David Chanhan Yun-Ju
Hseu Hu Chen Chen