Knobbe, Martens, Olson & Bear LLP
Key phosphorylation site of temperature sensitivity of influenza A virus and use thereof
A recombinant virus is obtained by mutating a codon that encodes a tyrosine residue at position 385 of NP protein in the genome of influenza A virus to a codon of phenylalanine residue. The virus WSN-Y385F is a temperature-sensitive virus that can normally replicate and survive at 37° C., and cannot normally replicate and cannot survive at 33° C. Phosphorylation of a NP protein of influenza A virus can be inhibited by mutating an amino acid residue at position 385 from N terminal of the NP protein of influenza A virus, from a tyrosine to a phenylalanine. The recombinant virus can be used in analyzing mechanisms of infection by influenza virus, and in connection with methods of prevention and treatment of infection by influenza virus.
Institute of Microbiology, Chinese Academy of Sciences
A C C A C C C A C
A61K C07K C12N A61P C12N C12N C12N A61K C12N
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Weinan Jing Wenjun
Zheng Li Liu