Barnes & Thornburg
Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis
Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.
Idaho Technology University of Utah Research Foundation
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Andrew O. Carl T. Brian E. Wade Jian Noriko
Crockett Wittwer Caplin Stevenson Chen Kusukawa