Birch, Stewart, Kolasch & Birch
Basophil-binding monoclonal antibody, method for separation of basophils, method for chemical mediator release from basophils, and method for testing release of basophil-derived chemical mediators
 The monoclonal antibodies of the present invention makes it possible to separate basophils suitable for the IgE-mediated specific chemical mediator release test, because it retains its reactivity with basophils even after being immobilized onto a solid carrier, and because it does not inhibit release of chemical mediators induced by allergens or anti-IgE antibody, and does not induce nonspecific release of chemical mediators. Also, the method for separating basophils of the present invention simplifies the separation of basophils from blood, and by using this method, the histamine release test which otherwise requires complex procedures can be simplified. Further, the group of cells obtained by the method for separating basophils of the present invention can easily be utilized in the release tests for chemical mediators released from basophils such as leukotriene and PAF, which otherwise require expertise for handling. 
Shionogi & Co., Ltd.
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C07K G01N G01N
inventional inventional inventional
Masaji Shinji Hiroshi
Nishimura Nishimura Nishi