Fish & Richardson P.C.
Bioconversion of cephalosporin C to glutaryl-7-aminocephalosporanic acid
 A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism. 
Food Industry Research and Development Institute
C
C12P
inventional
Wen-Shen Yun-Huey Wen-Hwei
Chu Lee Hsu