Dickinson Wright PLLC
Quick and sensitive method of quantifying mycolic acid to develop anti-microbial agents and a diagnostic kit thereof
The present invention relates to a rapid, sensitive, simple, and cost-effective spectrophotometric method of detecting and quantifying mycolic acid in a mycolic acid-fuschin dye complex with absorbance maxima ranging between 490-500 nm in the presence of various test compounds, for screening mycolic acid biosynthesis inhibitors useful as anti-microbial agents and a diagnostic kit thereof comprising basic fuschin dye in the concentration ranging between 0.1-1.0 gm/100 ml, phenol and 95% ethanol in the ratio ranging between 1:4 to 2:1 (v/v), and phenol and distilled water in the ratio ranging between 1:14 to 1:25.
Council of Scientific and Industrial Research
C Y
C12Q Y10S
inventional additional
Ajit Kumar Suman Preet Singh Tiruppadiripuliyur Ranganathan Santha Suchi
Shasany Khanuja Kumar Srivastava