Nath & Associates PLLC
Process for in vitro selection of high methol producing genotypes
The invention provides a rapid in vitro method for selection of menthol rich mint genotypes from a large population of independent clones, said method comprising the steps of (i) raising a heterogeneous population of Mentha arvensis clones in vitro or by vegetative methods, (ii) transferring the plantlets/shoots to a basal medium, containing cytotoxic compounds in an amount sufficient to cause toxic effect in more than 95% of the clones, (iii) selecting surviving clones, their hardening in the glasshouse, transfer to field, reconfirmation of menthol tolerance through repeated in vitro assays, and (iv) multiplying selected clones and confirming genetic uniformity through RAPD analysis.
Council of Scientific&Industrial Reaearch
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A01H A01H A01H
inventional inventional inventional
Dharmendra Arif Ali Suman Preet Singh Sunita Ajit Kumar Sushil Tiruppadiripuliyur Ranganathan Santha Mahendra Pandurang
Saikia Naqui Khanuja Dhawan Shasany Kumar Kumar Darokar