Pillsbury Winthrop Shaw Pittman LLP
Microbial production of 3-hydroxypropionic acid
A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification.
TECHNICAL UNIVERSITY OF DENMARK
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Jerome Kanchana Rueksomtawin Irina Niels Bjerg Jochen Jens Yun
Maury Kildegaard Borodina Jensen Förster Nielsen Chen