IPro, PLLC
Asparaginase mutant with efficient expression, activity and stability
The present disclosure discloses an asparaginase mutant with efficient expression, activity and stability, belonging to the technical fields of gene engineering and enzyme engineering. The amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101; meanwhile, compared with the wild type asparaginase mutant, the extracellular enzyme activity of the asparaginase fusion enzyme mutant is increased by up to 2.25 times, the stability is increased by up to 3.56 times, and the specific enzyme activity is increased by up to 1.34 times.
JIANGNAN UNIVERSITY
C C C C C
C12N C12Y C12N C12N C12N
inventional additional inventional inventional inventional
Jian Weixin Song Guocheng
Chen Zhao Liu Du