Leydig, Voit & Mayer
Pseudomonas exotoxin A with less immunogenic B cell epitopes
The invention provides a Pseudomonas exotoxin A (PE) comprising an amino acid sequence having a substitution of one or more of amino acid residues E420, D463, Y481, L516, R563, D581, D589, and K606, wherein the amino acid residues are defined by reference to SEQ ID NO: 1. The invention further provides related chimeric molecules, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions. Methods of treating or preventing cancer in a mammal, methods of inhibiting the growth of a target cell, methods of producing the PE, and methods of producing the chimeric molecule are further provided by the invention.
The United States of America, as represented by the Secretary, Department of Helath and Human Services
C C A C C C C C A C A C A C
C07K C07K A61K C07K C07K C07K C07K C07K A61K C07K A61K C07K A61P C07K
inventional additional additional inventional inventional inventional inventional additional inventional additional inventional additional inventional additional
Ira H. Masanori Wenhai
Pastan Onda Liu