Lackenbach Siegel, LLP
Seed-specific expression vector and its construction methods and applications
A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.
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C07K C12N
inventional inventional
Xiqing Yunyu Chenggang Kunsheng Xin Haimin Xue Tiemei Shengjun Zhan Haigang Liangyuan Pei
Chai Chen Yu Wang Wen Xu Li Shao An Jiao Lu Hu Liu