New England Bio Labs, Inc.
High fidelity restriction endonucleases
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
C C C G C G C C
C12Q C12Y C12N G01N C12Q G01N C12Q C12N
inventional inventional inventional additional inventional additional inventional inventional
Xianghui Shengxi Siu-hong Zhenyu Shuang-Yong Xuhui Chunhua Aine Dapeng Yishu
Li Guan Chan Zhu Xu Lai Zhang Quimby Sun Huang