Hoffman Warnick LLC
Method and composition using a quaternary ammonium compound
A method (e.g. high resolution melting analysis) of determining of the presence or absence of a target nucleic acid in a sample, e.g. a biological sample, the method comprising steps of:⋅(a) contacting the sample with a composition comprising⋅(i) a quaternary ammonium compound, e.g. 3-(trimethoxysilyl) propyl-dimethyl octadecyl ammonium chloride (3-TPAC) or a precursor thereof, in order to release the nucleic acids from said sample; and⋅(b) contacting the sample with a probe which is capable of interacting with the nucleic acid sequence; and⋅(c) observing and/or measuring a property of the sample, e.g. fluorescence from a dsDNA binding dye.
ARCIS BIOTECHNOLOGY HOLDINGS LIMITED
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inventional inventional additional additional inventional additional
Carlos Paul Jan
Toro Rueda Reeves Rogers