The Webb Law Firm
Cutinase-producing genetically engineered microorganism and use thereof
The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.
Jiangnan University
C C
C12N C12Y
inventional inventional
Jian Dan Jing Lei
Chen Wu Wu Wang