SZDC Law P.C.
Recombinant Bacillus subtilis with improved 2′-fucosyllactose production, and construction method thereof
The present invention provides a recombinant Bacillus subtilis with improved 2′-fucosyllactose production, and a construction method thereof. In the present invention, a strain capable of efficiently synthesizing 2′-fucosyllactose is obtained by the fusion expression of the fucosyltransferase gene and the L-fucokinase/guanosine 5′-diphosphate-L-fucose pyrophosphorylase gene in Bacillus subtilis BSGL-FF, the fermentation supernatant of which comprises a cumulative amount of 2′-fucosyllactose as high as 1.62 g/L, which is 55% higher than the amount achieved with the control strain. The construction method of the recombinant Bacillus subtilis of the present invention is simple, and convenient to use, and thus has good application prospects.
JIANGNAN UNIVERSITY
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Jieying Jianghua Jian Guocheng Long Ke Xueqin
Deng Li Chen Du Liu Liu Lv