The PL Law Group, PLLC
Method for labeling exosomes with radioactive substance and use thereof
A method for labeling exosomes with a radioactive substance using an amine group on surfaces of the exosomes includes providing a cell-derived exosome, treating a surface of the exosome with N-hydroxysuccinimide-azadibenzocyclooctyne (NHS-ADIBO), and mixing the treated exosome with N3-introduced chelator-radioactive substance to conduct a reaction between the chelator and an amine group present on the surface of the exosome, wherein the radioactive substance is introduced inside the exosome by the above reaction. The exosomes can be stably labeled at high labeling efficiency, and the exosomes can be favorably used as an agent for nuclear medicine imaging and therapeutic imaging for confirming the biological distribution of exosomes and whether the exosomes move to target organs and target diseases in animals including a human being.
SNU R&DB FOUNDATION POSTECH ACADEMY—INDUSTRY FOUNDATION
G A A
G01N A61K A61K
inventional inventional inventional
Yun-Sang Su Chul Do Won Hongyoon Dong Soo Jae Min Yong Song
Lee Jang Hwang Choi Lee Jeong Gho