SZDC Law P.C.
Method for improving GlcNAc production of recombinant bacillus subtilis
The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.
JIANGNAN UNIVERSITY
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C12N C12Y C12N C12N C12Y C12Y C12P
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Jianghua Long Yawen Yang Yang Jieying Guocheng Jian
Li Liu Zhao Song Gu Deng Du Chen