Hunton & Williams LLP
Functional assays for identifying compounds that modulate T1R1/T1R3 (umami) taste
Functional assays for identifying compounds that activate or modulate the activation of the T1R1/T1R3 (umami) taste receptor are provided. These assays detect the effect of one or more compounds on the activation of T1R1/T1R3 (umami) taste receptor or on the activation of T1R1/T1R3 umami taste receptor by another compound e.g., monosodium glutamate, lactisole, or another unami taste modulator. These assays preferably are cell-based functional assays and typically use cells, e.g., HEK-293 cells that express a G protein such as Gα15, Gα16 or gustducin. Compounds identified in the disclosed functional assays are potentially useful as flavor additives in compositions for human or animal consumption.
Senumyx, Inc.
G G G C A G G C C C G G A C
G01N G01N G01N C12Q A61P G01N G01N C07K C12Q C12Q G01N G01N A01K C07K
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Fernando Lena Mark Sergey Jon Elliot Shawn Hong Xiaodong
Echeverri Staszewski Zoller Zozulya Adler O'Connell Xu Li