Kilpatrick Townsend & Stockton LLP
Flowcell systems for single molecule detection
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
Pacific Biosciences of California, Inc.
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Nara Pamela Jiyan Gregory R Bambi L. Dan John G. K.
Narayanan Sheaff Chen Bashford Reynolds Draney Williams