Schmeiser, Olsen & Watts, LLP
Method of anaerobic tissue-targeted gene expression initiated by alcohol dehydrogenase promoter and the application thereof
A proteomic screening method for anaerobic-specific and expression-effective promoter, and a method of specially delivering and selectively stably expressing target gene in anaerobic tissue by an alcohol dehydrogenase promoter and uses thereof. The latter comprises an anaerobically-induced alcohol dehydrogenase promoter which is used as target gene promoter, anaerobic target bacteria and low copy number plasmid. Therefore, the target gene can be specially and highly expressed under hypoxia condition in vivo or in vitro. The selective gene expression which is driven by the alcohol dehydrogenase promoter in anaerobic tissue can be used as gene therapy method to treat anaerobic tissue disease including tumor, or to prepare anti-tumor drug.
NANJING UNIVERSITY
C C A
C12N C12N A61P
inventional inventional inventional
Hongqin Jianxiang Dongping Zichun
Zhuang Chen Wei Hua