Lee & Hayes, P.C.
Aspartase mutant, recombinant expression vector and recombinant bacterium containing aspartase mutant, and use thereof
The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a β-amino acid, and providing a practical and effective strategy for industrial production of the β-amino acid.
JIANGNAN UNIVERSITY
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C12Y C12N C12P C12N
inventional inventional inventional inventional
Jiamin Anqi Zhiming Xian Taowei Yaling Shuping Meijuan Minglong Jianing Meiqi
Chen Peng Rao Zhang Yang Wang Xu Xu Shao Zhang Wu