IPro, PLLC
Recombinant E. coli and method of producing Danshensu by using same
The present disclosure discloses a production method of Danshensu, belonging to the technical field of bioengineering. The present disclosure constructs a novel genetic engineering strain co-expressed by three enzymes, which can be applied to the production of optically pure 3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid. All of the (D/L)-α-hydroxycarboxylic acid dehydrogenase selected by the present disclosure have the characteristics of poor substrate specificity and strong optical specificity, and can produce optically pure D-danshensu and L-danshensu. Further, the production efficiency of the recombinant strain is improved by knocking out or enhancing the expression of a related gene on the E. coli genome to promote substrate transport and reduce product decomposition. The method for producing Danshensu and α-ketoglutaric acid by using the transformation of the recombinant strain according to the present disclosure is simple, has easily available raw materials, few impurities, and has good industrial application prospects.
Jiangnan University
C C C C C C C C C C C
C12N C12Y C12Y C12N C12P C12N C12Y C12N C12Y C12N C12Y
inventional additional additional inventional inventional inventional additional inventional additional inventional additional
Yajun Tianzhen Yujie Xiaohui Yanrui Jinbin
Bai Xiong Cai Zheng Ding Liu