Ketol-acid reductoisomerase using NADH
Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
Butamax Advanced Biofuels LLC
C C C C Y C C C C C C Y
C12N C12P C12N C12N Y02E C12N C12Y C12Q C12N C12N C12P Y02P
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Daniel P. Der-Ing Yougen Mark J.
Okeefe Liao Li Nelson