Hultquist, PLLC
Genetic marker for discriminating and detecting causative bacteria of fish Edwardsiellosis and Streptococcosis, and method for discriminating and detecting causative bacteria using same
The present disclosure relates to genetic markers for discrimination and detection of bacteria causing Edwardsiellosis and Streptococcosis in fish, and a method for discriminating and detecting the bacteria using the same. A genetic marker for discrimination and/or detection of each of Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis and Lactococcus garvieae, which cause fish diseases, is selected and a peptide nucleic acid and a primer pair, which are specific for the genetic marker, are used to amplify and obtain melting curves having different fluorescences depending on bacterial species. Thus, bacteria that cause fish diseases can be discriminated and whether or not fish would be infected with the bacteria can be detected in a simple, rapid and accurate manner.
NATIONAL INSTITUTE OF FISHERIES SCIENCE
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Hyun-Ja Hye Sung Myoung Sug Sung-Hee Jeung-Wan Jin-Do
Han Choi Kim Jung Do Kim