Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
Neutralizing agent for clostridium bacterial neurotoxins and preparation method thereof
Disclosed is a neutralizing agent for a clostridium bacterial neurotoxin and a preparation method thereof. The neutralizing agent contains a flavonol glycoside (A) or a flavonol glycoside (B). The flavonol glycoside (A) has a flavone skelton which has two hydroxyl groups at the 5 and 7 positions, at least one hydroxyl group bonding at either one of the 3′, 4′ and 5′ positions, and an ether linkage forming glycoside at the 3 position with a carbohydrate chain containing a rutinose skelton. The flavonol glycoside (B) has also another ether linkage at the 4′ position with a carbohydrate chain containing rhamnose. The neutralizing agent is prepared by separating the thearubigin fraction of black tea extract by elution with methanol/water solvent using a reverse phase liquid chromatography. A fraction eluted with methanol/water solvent containing 40% methanol and a fraction eluted with methanol/water solvent containing 60% methanol have high neutralizing activity, and at least one of them is collected.
Ito En, Ltd.
A A C A A A Y A
A61K A23V C07H A61P A23L A23L Y02A A61P
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Masakazu Shin-ichi Iwao
Nishimura Sawamura Sakane