Ketol-acid reductoisomerase using NADH
Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
Butamax(TM) Advanced Biofuels LLC
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Der-Ing Daniel P. Mark J. Yougen
Liao Okeefe Nelson Li