IPro, PLLC
Multicopper oxidase mutant with improved salt tolerance
The present disclosure provides a multicopper oxidase mutant with improved salt tolerance. Threonine at site 317 of wild-type multicopper oxidase WT was mutated to asparagine, leucine at site 386 was mutated to tyrosine, and serine at site 427 was mutated to glutamic acid by site-directed mutagenesis to obtain a mutant T317N-L386Y-S427E. Compared with WT, the tolerance of T317N-L386Y-S427E to 6%, 9%, 12%, 15% and 18% NaCl (W/V) is improved.
JIANGNAN UNIVERSITY
C C A A
C12N C12N A23L A23L
inventional inventional inventional inventional
Guocheng Jian Jie Tao Fang Jingwen
Du Chen Xu Yang Fang Zhou