McAulay Fisher Nissen Goldberg & Kiel, LLP
Immunoassay element and process and immunoassay
 An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element. 
Fuji Photo Film Co., Ltd. Fujirebio Inc.
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Tadashi Naofumi Yukio Toshihiro Yoshihiko Yoshihiro Tetsuji Toshikage
Ninomiya Hora Sudo Mori Makino Ashihara Tanimoto Hiraoka