Abelman, Frayne & Schwab
Isolated bacterial strain of Achromobacter sp. MTCC 5605 and a highly enantioselective epoxide hydrolase isolated therefrom
The present invention relates to a novel epoxide hydrolase enzyme which aims to achieve a high degree of resolution towards a broader range of substrates with high enantioselectivity and yields with minimal product inhibition. The invention further relates to a new bacterial strain Achromobacter sp. MTCC 5605 isolated from a petroleum-contaminated sludge sample, capable of producing the said enzyme. It is notable that the enzyme can be used as whole bacterial cell preparation, which allows continuous hydrolysis of substrates at even higher concentration and have an advantage of being recycled. The invention further relates to a process for the hydrolysis of different aryl epoxides which are potential synthons of intermediates for the synthesis of chiral amino alcohols and bioactive compounds like β-blockers.
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
C C C C C C
C12Y C12P C12N C12N C12R C12P
inventional inventional inventional inventional additional inventional
Ahmed Anver Basha Matam Shiva Rohini Chityal Ganesh
Kamal Shaik Kumar Khanna Kumar