Foley & Lardner LLP
Glycoprotein and process for producing the same
A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with α-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.
National Institute of Advanced Industrial Science and Technology Tokyo Metropolitan Organization for Medical Research
A A A A A A A C A C A
A61P A61P A61K A61K A61P A61P A61P C12N A61P C12P A61P
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Yoshifumi Kazuo Hitoshi Yasunori Makoto
Jigami Kobayashi Sakuraba Chiba Takeuchi