JCIP Global Inc.
Pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus
The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.
OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF
 AGRICULTURAL SCIENCES
C C G C G G G C G
C12N C12N G01N C12N G01N G01N G01N C12P G01N
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Liangxiao Peiwu Xiupin Xiaoxia Xuefang Wen Qi Huali
Zhang Li Wang Ding Wang Zhang Zhang Xie