Processing proteins from gels for analysis using mass spectrometry
The invention relates to methods and devices used for digesting small amounts of protein in tiny cut gel pieces and for extracting the peptides resulting from the digestion in preparation for analysis by mass spectrometry.The invention involves digesting proteins using enzymes within the gel pieces in vessels which have permeable but lyophobic bases in such a manner that they scarcely touch the walls of the vessel, and then rapidly removing the digested proteins from the gel pieces almost completely by gentle centrifuging. It is then advantageous to bond the peptides reversibly to suitable surfaces as quickly as possible. For this purpose, the bases of the vessels may contain structures for bonding the peptides which are suitable for washing and subsequently eluting the peptides. A number of vessels can be combined together to form plates which, for example, can have the size of microtiter plates.
Bruker Daltonik GmbH
B G
B01L G01N
inventional additional
Helmut Eckhard Martin
Meyer Nordhoff Schurenberg