Klarquist Sparkman, LLP
Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
The United States of America as represented by the Department of Health and Human Services
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C12N C12N
inventional inventional
Donald L. Xin-tian Jian-Dong Depei Nina
Court Li Huang Liu Costantino