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TMV resistant tobacco plant containing short N introduced fragment and method for breeding same
A TMV resistant tobacco plant containing a short N introgressed segment and a method for breeding the same. A homozygous tobacco plant containing an N introgressed segment is hybridized with a tobacco plant of genotype nn to obtain an F1 progeny tobacco plant of genotype Nn. The F1 progeny tobacco plant is hybridized with the tobacco plant of genotype nn, to obtain population materials for screening to obtain the short N introgressed segment. TMV is inoculated at a seedling stage, and Nn genotype plants showing necrotic lesion are obtained by screening the population materials. The Nn genotype plants are genotyped using a molecular marker TN5.51 primer pair and an N gene-specific molecular marker N1N2 at the right end of the N introgressed segment. A plant found to be negative when tested by the TN5.51 primer pair and to be positive when tested by the N1N2 molecular marker is a plant comprising the short N introgressed segment. The size of non-target genomic components deleted from plants containing the short N introgressed segment is estimated using TN5.34 and TN5.20 and TN4.99 primer pairs. The obtained short N introgressed segment is applicable to germplasma innovation and breeding of TMV resistant tobacco. The invention is helps to reduce linkage drag with the N gene.
YUNNAN ACADEMY OF TOBACCO AGRICULTURAL SCIENCES
C A A C A A
C12Q A01H A01H C12N A01H A01H
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Yongping Changjun Yong Haiqin
Li Huang Liu Yu