IPro, PLLC
Multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins
The present invention relates to a multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins, which relates to the field of enzyme engineering and protein purification. The present invention provides a self-assembling amphipathic peptide, which fused with proteins including alkaline polygalacturonate lyase (PGL), lipoxygenase (LOX) and green fluorescent protein (GFP), which leads to successful purification by the nickel affinity chromatography with recovery rates were 47.01%, 39.01% and 56.1%, respectively. Furthermore, the expression quantity and thermostability of the three proteins were enhanced in different degree. Of which the half-life of the PGL-S1v1 and LOX-S1v1 were 2.3 and 3.8-fold as compared with the corresponding wild-type and the specific activity were 1.1 and 1.9-fold increase, respectively. The crude enzyme activity of PGL-S1v1 was 9-fold increase than the PGL.
JIANGNAN UNIVERSITY
C C C C C C C C
C12Y C12N C12Y C07K C07K C07K C07K C12N
inventional inventional inventional inventional inventional additional additional inventional
Jian Weixin Guocheng Song
Chen Zhao Du Liu