Lawson & Weitzen, LLP
Method of screening for a substance that strengthens a bond between thioredoxin and macrophage migration inhibition factor
Disclosed is a screening method which can select a substance having an influence on the binding between thioredoxin and MIF. The screening method is characterized in that a test substance capable of enhancing the binding between a polypeptide belonging to the thioredoxin family and a macrophage migration inhibitory factor is selected through the steps of mixing at least one binding substance selected from the substances (1) to (4) shown below with the test substance, allowing the binding substance to bind to the macrophage migration inhibitory factor, and confirming the binding between the binding substance and the macrophage migration inhibitory factor: (1) a polypeptide belonging to the thioredoxin family; (2) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues in an amino acid sequence for a polypeptide belonging to the thioredoxin family and has the same activity as that of a polypeptide belonging to the thioredoxin family; (3) a gene encoding the polypeptide (1); and (4) a gene encoding the protein (2).
Redox Bioscience, Inc.
A A A A A A C A A A A C A A A A A A A
A61P A61P A61P A61P A61P A61P C12N A61K A61P A61P A61P C12Y A61P A23L A61P A61P A61P A61K A61P
inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional inventional
Junji Tomohisa Noriko Aoi Norihiko Tomijiro
Yodoi Horibe Kato Son Kondo Hara