Revolution IP, PLLC
Method for preparing DNA library derived from FFPE tissue using endonuclease
The present invention relates to a method of preparing a DNA library derived from FFPE tissue using endonucleases, and the majority of general clinical samples are FFPE tissues and in case of old FFPE tissues, DNA library preparation often fails and NGS analysis becomes difficult and therefore there is a desperate need to overcome these problems. S1 nucleases are enzymes which specifically cleave only single-stranded DNA and in almost all FFPEs, there is a nick in the dsDNA everywhere, which can be the target of S1 nucleases. In the process of extracting gDNA from FFPE tissues, since when S1 nucleases are treated and eluted under appropriate conditions during elution, a DNA fragment can be obtained with DNA having a size suitable for DNA library preparation together with the DNA extraction, the Covaris fragmentation process can be omitted and cost and time can be reduced.
GenoPeaks Co., Ltd.
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C12N C12Q C12Q C40B C40B C12N
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Hye-Joon Sung-Min Ji-Young Yu Jin Se-Jin Chang Ohk
Jeon Chun Lee Kim Jang Sung