Optimized method for antibody capturing by mixed mode chromatography
Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.
Hoffmann-La Roche Inc.
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inventional inventional inventional
Markus Roberto Thorsten Hidenari
Strasser Falkenstein Lemm Yamada