IPro, PLLC
Method for increasing citric acid production by Aspergillus niger fermentation
The invention discloses a method for improving citric acid production by Aspergillus niger fermentation, which integrates Aspergillus niger GABA pathway succinate semialdehyde dehydrogenase SSD gene into Aspergillus niger genome to obtain recombinant Aspergillus niger strain, and uses recombinant black The Aspergillus strain ferments to produce citric acid; the expression of the succinate semialdehyde dehydrogenase SSD gene is regulated by the Pgas promoter. The method of the invention realizes the expression of succinate semialdehyde dehydrogenase SSD in Aspergillus niger to enhance the GABA pathway so as to strengthen the TCA cycle and promote the synthesis of citric acid.
JIANGNAN UNIVERSITY
C C C C
C12P C12N C12N C12Y
inventional inventional inventional inventional
Long Zhijie Fuxin Xiaodong Jian Guocheng Cheng Jianwei Sai Jianghua Xian
Liu Hu Sun Jiang Chen Du Zhang Jiang Jin Li Yin