Burns, Doane, Swecker & Mathis, L.L.P.
Method for producing phytase
 A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase. 
Mitsui Chemicals, Inc.
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C12N C12N C12P
inventional inventional inventional
Daisuke Takeshi Junko Tadashi Yasuyoshi Hitoshi Nobuo
Mochizuki Nakamura Tokuda Suzuki Sakai Takahashi Kato