Finnegan, Henderson, Farabow, Garrett & Dunner, LLP
Methods for accurate sequence data and modified base position determination
Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
Industrial Technology Research Institute
Jenn-Yeh Hui-Ling Hung-Chi Chung-Fan Chao-Chi
Fann Chen Chien Chiou Pan