Ketol-acid reductoisomerase using NADH
Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
Butamax Advanced Biofuels LLC
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inventional inventional additional
Der-Ing Yougen Daniel P. Mark J.
Liao Li Okeefe Nelson