Rothwell, Figg, Ernst & Manbeck P.C.
Bacterial isolates of the genus Klebiella, and an isomaltulose synthase gene isolated therefrom
Two strains of a novel bacterial species, Klebsiella singaporensis LX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity.
Agency for Science, Technology and Research
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C12Y C12P C12R C12P C12N C12N
inventional inventional additional inventional inventional inventional
Daohai Xianzhen Lian Hui
Zhang Li Zhang