Knobbe Martens, Olson & Bear LLP
Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.
Illumina, Inc.
C C C C C C C C
C12Q C12Q C12Q C12Q C12Q C12Q C12Q C12Q
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Kay Liangliang Lea Tarun Kevin L. Boyan Cyril
Klausing Qiang Pickering Khurana Gunderson Boyanov Delattre