McCoy Russell LLP
Preparation method of adiposomes, and use thereof
A preparation method of adiposomes, and use thereof. Provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising a1) vortexing phospholipid and neutral lipids in a buffer, centrifuging the resulting mixture, and collecting an upper liquid phase; a2) purifying the upper liquid phase twice or more by uniformly mixing the upper liquid phase with the buffer, layering the mixture, and collecting an upper liquid phase; and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in containing adiposomes. For the adiposomes prepared by the method, one or more resident proteins and/or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins; and they all play important roles in preparing drugs and/or drug carriers.
INSTITUTE OF BIOPHYSICS, CHINESE ACADEMY OF SCIENCES
A A C A A A
A61K A61K C07K A61K A61K A61K
inventional inventional inventional inventional inventional inventional
Yang Pingsheng Xuejing Xiaoming Chang
Wang Liu Ma Zhou Zhou