Choate, Hall & Stewart
Adjustable threshold switch
Provided are methods and compositions for regulating gene expression in a cell. The invention provides recombinant adjustable-threshold switches which contain a first inducible promoter-regulatory gene operon and a second constitutive promoter-regulatory gene operon. A threshold amount of activating agent is required to switch the inducible promoter &#8220;on&#8221; and the constitutive promoter &#8220;off&#8221;. An adjustable-threshold switch is useful to reversibly switch the expression of a gene of interest between a stable &#8220;on&#8221; state and stable &#8220;off&#8221; state in response to an activating agent at or above a threshold concentration.
Cellicon Technologies, Inc.
C C
C12N C12N
inventional inventional
Timothy James J.
Gardner Collins