Foley & Lardner
&bgr;1-4 N-acetylglucosaminyltransferase and gene encoding
The present invention relates to a novel enzyme having &bgr;1&#8594;4 N-acetylglucosaminyltransferase (GnT-IV) activity; a gene encoding the enzyme; a recombinant DNA comprising the gene; a host cell comprising the recombinant DNA; a method for producing an enzyme protein having GnT-IV activity comprising culturing the host cell in a medium; and a saccharide in which the sugar chain is modified using a GnT-IV.According to the present invention, a novel GnT-IV, a method for producing the enzyme and a gene coding for the enzyme are provided. With the GnT-IV of the present invention, it has become possible to produce a saccharide having a branching structure which could not be formed with conventional glycosyltransferases. Thus, the GnT-IV of the invention is useful not only for producing or improving glycoconjugate type pharmaceuticals, reagents and foods, but also for modifying the sugar chain structure of any biopolymer.
Kirin Beer Kabushiki Kaisha
C A
C12N A61P
inventional inventional
Naoyuki Aruto Suguru Mari Makoto
Taniguchi Yoshida Oguri Minowa Takeuchi