Oblon, Spivak, McClelland, maier & Neustadt, P.C.
Method for producing nucleoside-5'-phosphate ester
 A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided. 
Ajinomoto Co., Inc.
C C
C12P C12N
inventional inventional
Hideaki Yasuhisa Yasuhiro Takashi
Yamada Asano Mihara Utagawa