Method for high-efficiency production of pinoresinol using an H2O2 auto-scavenging cascade
The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.
Jiangnan University
C C C A C A C C C C Y
C12N C12N C12N A61P C12N A61P C12Y C12P C12Y C12P Y02P
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Jingwen Guocheng Jian Yongkun
Zhou Du Chen Lv