Hunton & Williams LLP
Cell lines that stably or transiently express a functional sweet (T1R2/T1R3) taste receptor
Cells are engineered to stably or transiently express T1R2 and T1R3 polypeptides, desirably in association with a G protein. The resultant heteromeric taste receptor is a sweet taste receptor and responds to sweet taste stimuli such as artificial and natural sweeteners, e.g., saccharin. The invention provides a preferred mammalian cell lines, e.g., HEK-293 cells that stably express T1R2/T1R3 and Gα15 under inducible conditions. These cells are useful in cell-based assays for identifying compounds that elicit or modulate sweet taste.
Senomyx, Inc.
G C G C A G G C A G G C C G
G01N C12Q G01N C12Q A61P G01N G01N C07K A01K G01N G01N C07K C12Q G01N
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Shawn Lena Hong Fernando Xiaodong Mark Jon Elliot Sergey
O'Connell Staszewski Xu Echeverri Li Zoller Adler Zozulya