Greenlee, Winner and Sullivan, P.C.
Xylanase obtained from an anaerobic fungus
 A xylanase gene, denoted xynC, encoding a novel xylanase (XynC) obtained from the anaerobic fungus Neocallimastix patriciarum is provided. The DNA sequence of the xynC gene is also provided. Transformation of microbial and plant hosts with the xynC gene is described. The xynC gene may be used to design probes for use in hybridization experiments to isolate xylanase genes from other anaerobic fungi. The xynC gene has been used to construct an oleosin-xynC expression construct encoding an oleosin-xylanase fusion protein which retains xylanase activity. Transgenic Brassica napus (canola), transformed with the oleosin-xynC expression construct, expresses the oleosin-xylanase fusion protein which is immobilized in the oil-body membrane of the B. napus seeds. Canola meal, the protein-rich residue left after canola oil is extracted from canola plants, when derived from the transgenic B. napus of the present invention, retains substantial xylanase activity, making it an ideal animal feed supplement. 
Her Majesty the Queen in Right of Canada, as represented by the Department of Agriculture and Agri-Food
C C C C C C
C12N C07K C12N C12N C12Y C07K
inventional additional inventional inventional inventional inventional
Leonard B. Cecil Wallace Youji Jin-Hao Kuo-Joan Maurice M.
Selinger Forsberg Hu Liu Cheng Moloney