Parker Highlander PLLC
Nitrilase from arabis alpina, its encoding gene, vector, recombinant bacterial strain and uses thereof
The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.
Zhejiang Chiral Medicine Chemicals Co. Ltd. Zhejiang University of Technology
C C C
C12Y C12P C12N
inventional inventional inventional
Jianfeng Qin Youming Yuguo Weirong Renchao Tianchun
Weng Zhang Huang Zheng Fan Zheng Liu