Christie, Parker & Hale, LLP
Process for seperating alpha-1-proteinase inhibitor from COHN IV.sub.1 +1V.sub.4 paste
 The present invention is directed to a process for purifying alpha-1-PI. The process comprises providing an impure protein fraction which comprises alpha-1-PI. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8.5. PEG is added to precipitate .alpha.-2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl.sub.2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchange medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD.sub.280. 
Alpha Therapeutic Corporation
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C07K C07K Y10S Y10S
inventional inventional additional additional
Mark Hirokazu Kazuo Lyndon Evelyn Duk S.
Lepe Ito Takechi Luz Nario Hwang