JMB Davis Ben-David
Method for enzymatic deacidification of polyunsaturated fatty acid-rich oil
An enzymatic deacidification method for partial glyceride lipase and PUFA-rich oil, comprising the following steps: 1) mixing a polyunsaturated fatty acid (PUFA)-rich oil with a non-polar organic solvent and a short-chain monohydric alcohol, adding an immobilized partial glyceride lipase to carry out an esterification reaction, wherein the partial glyceride lipase is a mutant obtained by mutating the Phe at the 278th position of Lipase SMG1 as Asn; 2) recovering the immobilized enzyme, and recovering the organic solvent and the monohydric alcohol so as to obtain a deacidified PUFA-rich oil. The partial glyceride lipase does not catalyze alcoholysis of triglyceride and like side reactions, has high deacidification efficiency, low reaction temperature, prevents high temperature oxidation of PUFAs, and the immobilized enzyme may be recovered and reused repeatedly, and thus the present invention has good application prospects in industry.
SOUTH CHINA UNIVERSITY OF TECHNOLOGY
C C C C C Y
C12N C12P C12P C12N C12Y Y02P
inventional inventional inventional inventional additional additional
Weifei Daoming Dongming Yonghua Bo Nan
Wang Li Lan Wang Yang Liu