Method for cultivation of Pseudomonas bacteria
 Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase. 
Nitto Kagaku Kogyo Kabushiki Kaisha Hideaki Yamada
C C Y
C12N C12N Y10S
inventional inventional additional
Hideaki Koitchiro
Yamada Ryuno