Ketol-acid reductoisomerase using NADH
Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
Butamax Advanced Biofuels LLC
C C C C Y C C C C C C Y
C12N C12N C12N C12Y Y02E C12P C12P C12N C12N C12Q C12N Y02P
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Mark J. Der-Ing Daniel P. Yougen
Nelson Liao Okeefe Li