Rabin & Berdo, P.C.
Preparation method of hydrogel based on decellularized tissue using supercritical fluid-organic solvent system
A method for preparing a decellularized tissue-based hydrogel with maximized ability of preserving various tissue-derived proteins, growth factors and cytokines by using a supercritical fluid-organic solvent system through (a) a step of decellularizing a biological tissue by bringing the same to contact with a supercritical fluid and an organic solvent at the same time; (b) a step of washing the decellularized tissue; (c) a step of preparing a decellularized tissue solution by mixing the washed decellularized tissue with one selected from an enzyme solution, an acidic solution and a mixture thereof; (d) a step of titrating the decellularized tissue solution to pH 5.5-7.8 by treating with a basic solution; and (e) a step of allowing the titrated decellularized tissue solution to stand at 30-40° C. and utilizing the same as a tissue engineering material with improved angiogenesis and tissue regeneration abilities.
Korea Institute of Science and Technology
A A C A A A C C A
A61L A61L C12Y A61L A61L A61L C12Y C12Y A61L
inventional inventional inventional inventional inventional inventional inventional inventional inventional
Young Mee Soo Hyun Yoo Jin
Jung Kim Seo