Hamilton, Brook, Smith & Reynolds, P.C.
In vitro metabolic engineering on microscale devices
Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
Rensselaer Polytechnic Institute Darpa The Regents of the University of California
B B B B B B B B B B B B B B B B B B B B B B B B
B01J B01J B01J B01L B01J B01J B01J B01J B01J B01L B01L B01J B01J B01J B01J B01J B01L B01J B01J B01J B01J B01J B01L B01J
additional additional additional additional additional additional additional additional additional inventional additional additional inventional additional inventional additional additional additional additional additional additional additional additional additional
Aravind David H. Jungbae Douglas S. Jonathan S.
Srinivasan Sherman Kim Clark Dordick