Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
Method for producing nucleoside-5&#8242;-phosphate ester
A method for producing nucleoside-5&#8242;-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phosphate group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5&#8242;-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.
Ajinomoto Co., Inc.
C C
C12P C12N
inventional inventional
Hideaki Yasuhiro Yasuhisa Takashi
Yamada Mihara Asano Utagawa