Antonelli, Terry, Stout & Kraus
Method for analyzing amino acids and apparatus therefor
 A method for analyzing amino acids in biological liquids wherein a buffer liquid including unknown components to be analyzed is introduced to a separation column for separating amino acids. After reaction of the buffer liquid in a reactor, the amino acids are detected by a photometer. The flow rate of the buffer liquid is maintained at a predetermined value until the asparagine, glutamine acid and glutamine are separated from the separation column and the flow rate of the buffer liquid is varied stepwise or in linear gradient after separation of the above three components from the separation column. 
Hitachi Instrument Engineering Co., Ltd. Hitachi, Ltd.
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Hiroshi Kohta Yoshio
Satake Kimiyoshi Fujii