Morrison & Foerster LLP
Method for isolating and purifying recombinant human serum albumin from transgenic rice grain
A method for separating and purifying recombinant human serum albumin (rHSA) from transgenic rice grain, sequentially comprising the steps of: 1) subjecting crude extract of rHSA to cation exchange chromatography to obtain primary product I; 2) subjecting the primary product I to anion exchange chromatography to obtain secondary product II; 3) subjecting the secondary product II to hydrophobic chromatography to obtain purified rHSA. The method may further comprise a step of ceramic hydroxyapatite chromatography prior to the hydrophobic chromatography. The method has the advantages of low cost and easy operation. The resultant rHSA has a purity of about 99% by HPLC.
Healthgen Biotechnology Co., Ltd.
C B C
C07K B01J C07K
inventional inventional inventional
Jingru Yang Guangfei Daichang
Liu He Li Yang