Townsend and Townsend and Crew LLP
Charge switch nucleotides
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
Pacific Biosciences of California, Inc.
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C07H C07H C12Q C07H C40B
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Pamela Bambi L. Dan Gregory R. John G. K. Nara Jiyan
Sheaff Reynolds Draney Bashford Williams Narayanan Chen