Jones Day
Method for refining protein including self-cutting cassette and use thereof
The present invention relates to a self-cleaving fusion protein including a target protein, a peptide consisting of amino acid sequence represented by LPXTG, a domain of Sortase A having cleaving function, and a tag, which are sequentially positioned from the amino terminal; a nucleic acid encoding the same; an expression vector including the nucleic acid of the present invention; and a cell transformed with the expression vector of the present invention. In addition, the present invention relates to a method for refining a target protein including culturing, dissolving, and purifying the transformed cell, and a method for preparing a therapeutic antibody-drug conjugate by using the purifying method.
AbTLAS CO., LTD.
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Hye In Byeong Doo Jee Sun Hyo Jung Eung-Suk
Kim Song Yun Choi Lee