Quarles & Brady LLP
Portable, low-cost pathogen detection and strain identification platform
Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.
MASSACHUSETTS INSTITUTE OF TECHNOLOGY ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY PRESIDENT AND FELLOWS OF HARVARD COLLEGE
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Melissa K. Dana Alexander Thomas James J. Keith Guillaume
Takahashi Braff Green Ferrante Collins Pardee Lambert