Duane Morris LLP
Purification of recombinant EV71 virus-like particle and method for preparing vaccine thereof
The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria; inducing expression of the EV71 virus-like particle protein expression using methanol; collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption; performing precipitation on the supernatant with ammonium sulfate; and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.
Beijing Minhai Biotechnology Co., Ltd.
A C C A A A G G A C A A
A61P C12N C12N A61K A61K A61K G01N G01N A61K C12N A61K A61K
inventional inventional additional inventional additional inventional inventional additional inventional inventional inventional additional
Lin Jiankai Lei Meirong Guoshun Jin Yingzhi Haifeng Gaimei
Guo Liu Chen Gu Li Li Xu Xiao Zhang