McDonnell Boehnen Hulbert & Berghoff LLP
Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom
Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.
Evolva S.A. Cargill, Incorporated
C C A A
C07H C12P A23L A23V
inventional inventional inventional additional
Ariene M. Ting Liu James C. Veronique Hans Peter Manuel Quiros Simon
Fosmer Carlson Anderson Douchin Smits Asensio Carlsen