Bozicevic, Field & Francis LLP
Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
Fuso Pharmaceutical Industries, Ltd. National University Corporation Hokkaido University
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inventional additional inventional
Yuzuru Yusuke Hiroshi Keiichi Yasuhiko
Kitahara Suzuki Tahara Yamamoto Suzuki