Browdy and Neimark
Novel restriction endonuclease SplI and process for the production of the same
 A novel restriction endonuclease SplI which has the following physicochemical properties: PA0 (1) recognizing the following base sequences in double-stranded deoxyribonucleic acid ##STR1## and cleaving said sequences in the phosphodiester bonds between C and G as indicated with the vertical arrows to produce DNA fragments having one strand comprising four bases at the 5'-terminal; PA0 (2) cleaving double-stranded deoxyribonucleic acid .lambda.-DNA in one position, Col El in two positions and .phi.x 174 RF in two positions; PA0 (3) being activated with 5 to 20 mM Mg.sup.2+ ; and PA0 (4) exhibiting an activity at a NaCl concentration of 0 to 200 mM; and a process for the production of the restriction endonuclease SplI which comprises culturing a restriction endonuclease SplI-producing alga belonging to the genus Spirulina, collecting the cells, obtaining a cell-free extract therefrom the separating and purifying the restriction endonuclease SplI. 
Dainippon Ink and Chemicals, Inc. Takara Shuzo Co., Ltd.
C
C12N
inventional
Masahide Teruo Akira Nobutsugu Keiko Masaki
Kawamura Watanabe Obauashi Hiraoka Kita Sakakibara