Bozicevic, Field & Francis LLP
Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
Fuso Pharmaceutical Industries, Ltd. National University Corporation Hokkaido University
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Yusuke Yasuhiko Hiroshi Keiichi
Suzuki Suzuki Tahara Yamamoto