Genetically engineered Yarrowia lipolytica with enhanced extracellular secretion of α-ketoglutarate
The present invention provides four keto acid transporter encoding sequences selected from 6611 protein coding sequences of Yarrowia lipolytica CLIB122 database. Also provided are recombinant Yarrowia lipolytica strains overexpressing the keto acid transporters, which have increased extracellular secretion of α-ketoglutarate. The present invention can be used to increase extracellular levels of α-ketoglutarate during the fermentation process and lower downstream purification cost for α-ketoglutarate production.
Jiangnan University
C C C C
C12N C12N C12P C07K
inventional inventional inventional inventional
Jian Guocheng Jingwen Hongwei
Chen Du Zhou Guo