Wenderoth, Lind & Ponack, L.L.P.
RNA in situ hybridization
A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.
CELISH FD, INC.
C C
C12Q C12Q
inventional inventional
Tatsuhiro Chizuru Masahiro Hirofumi
Nakano Nagayoshi Matsuoka Doi