New England Biolabs, Inc
High fidelity restriction endonucleases
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
New England Biolabs, Inc.
G C C C G C C C
G01N C12Q C12Q C12Y G01N C12N C12N C12Q
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Dapeng Shengxi Aine Shuang-Yong Zhenyu Xuhui Chunhua Yishu Xianghui Siu-hong
Sun Guan Quimby Xu Zhu Lai Zhang Huang Li Chan