Ostrolenk Faber LLP
Nanoparticle sensor for measuring protease activity and method for manufacturing the same
Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.
The Asan Foundation Futurechem Co., Ltd. Korea Institute of Science and Technology
Y Y Y G Y Y C
Y10S Y10S Y10S G01N Y10S Y10S C12Q
additional additional additional inventional additional additional inventional
Kyeong-Soon In-Chan Dae-Yoon Dae-Hyuk Kui-Won Seul-Ki Ick-Chan Kwang-Meyung Seung-Jin Seung-Jae
Park Youn Chi Moon Choi Lee Kwon Kim Lee Myung