Method for constructing high-resolution single cell Hi-C library with a lot of information
Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.
ZHEJIANG ANNOROAD BIO-TECHNOLOGY CO. LTD. ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD.
C C C C C C
C12Q C12Q C40B C12Q C40B C12N
inventional inventional inventional additional additional inventional
Junbin Hongmei Zhihua Chongjian Zhaoling Xiaolin Jiezhong Dawei
Liang Zhao Pei Chen Xuan Li Zhang Li