WPAT, P.C.
Blotting method for rapidly analyzing nucleic acid
The present invention relates to a blotting method for rapidly analyzing nucleic acid comprising the steps of transferring a nucleic acid to be analyzed to the substrate and fixing the nucleic acid to be analyzed absorbed on the substrate; directing adding a nucleic acid probe to hybridize in a short time, without blocking the areas where the nucleic acid to be analyzed has not been fixed; removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed by washing; and finally detecting the hybridization signal. According to the present invention, since the prehybridization is not needed and the hybridization and washing time is shortened, the time for the nucleic acid hybridization is dramatically shortened. Therefore, the whole blotting procedures for rapidly analyzing nucleic acid may be finished quickly.
C
C12Q
inventional
Chung-Cheng Jau-Der Pei-Tai
Chang Chen Chen