IPro, PLLC
Mutant with enhanced secretion of L-asparaginase and its application
The present invention provides a mutant with enhanced secretion of L-asparaginase and its application, which relates to the field of enzyme engineering. This invention successfully constructed a mutant with enhanced secretion of L-asparaginase through N-terminal deletion on amino acid sequence of L-asparaginase, and then expressed the mutant via a recombinant strain. Compared with the wild L-asparaginase, the secretion ability of L-asparaginase mutant in present invention significantly improves by 3.14 times compared to that of wild type. The recombinant strain that constructed in this prevention has an L-asparaginase yield of 407.6 U/mL and a production efficiency of 9.26 U/(mL/h), which reaches to the highest yield been reported.
Jiangnan University
A A C C
A61K A61P C12Y C12N
additional inventional inventional inventional
Yue Jie Hui Jian Hongye Yun Song Guocheng
Feng Ruan Gao Chen Cheng Jiao Liu Du