Klarquist Sparkman, LLP
Cancer stem cell population and method for production thereof
The purpose of the present invention is to provide: a cancer stem cell mass from which cells incapable of forming cancer are substantially removed and which has a characteristic property of reproducing a layered structure of a cancer tissue; a process for producing the cancer stem cell mass; and use of the cancer stem cell mass. For achieving the purpose, the present inventors grew a human cancer tissue repeatedly in a NOG mouse, separated cancer cells from the grown cancer tissue, and made a comparison of various cancer cell culture processes with each other. As a result, a cancer stem cell composition which is homogeneous and is substantially free of the coexistence of cells capable of forming cancer and cells incapable of forming cancer in a mixed state can be produced successively by employing an attached culture process using a serum-free stem cell culture medium rather than a generally employed floating culture process, and consequently the present invention has been accomplished.
CHUGAI SEIYAKU KABUSHIKI KAISHA
G C A G
G01N C12N A61P G01N
inventional inventional inventional inventional
Shinta Atsuhiko Yu Jau Koichi Tatsumi Hisafumi Masami
Kobayashi Kato Chen Matsubara Yamazaki Okabe Suzuki