Vedder Price P.C.
Lipid acyltransferase proteins and methods of making them
The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.
DUPONT NUTRITION BIOSCIENCES APS
C A A C
C12N A21D A23V C12N
inventional inventional additional inventional
Richard R. Jørn Dalgaard Marguerite A. Lone B. Charlotte Johansen Jens Frisbæk Janne Charlotte R. Charlotte Horsmans Lene B. Birgitte Ø. Morten K. Rikke H. Jørn Borch
Bott Mikkelsen Cervin Miller Vedel Sorensen Brunstedt Thoudahl Poulsen Jensen Wittschieben Larsen Lorentzen Søe