Hunton & Willims LLP
Recombinant methods for expressing a functional umami (T1R1/T1R3) taste receptor
Methods for expressing a heteromeric taste receptor that responds to umami taste stimuli are provided. These methods comprise the co-expression of T1R1 and T1R3 nucleic acid sequences in a host cell that desirably also expresses a G protein that couples therewith, e.g., Gα15 and Gα16 or gustducin. In preferred embodiments, the host cells will be mammalian cells or Xenopus oocytes. These nucleic acid sequences are expressed constitutively or under inducible conditions. In preferred embodiments, the methods will yield mammalian cells that stably express a T1R1/T1R3 umami taste receptor under inducible conditions, e.g., HEK-293 cells that express Gα15.
Senomyx, Inc.
C C G C C G G C A G G A G G
C07K C12Q G01N C07K C12Q G01N G01N C12Q A61P G01N G01N A01K G01N G01N
inventional additional additional additional inventional inventional additional additional inventional inventional additional additional inventional additional
Jon Elliot Hong Xiaodong Sergey Mark Shawn Femando Lena
Adler Xu Li Zozulya Zoller O'Connell Echeverri Staszewski