Fay, Sharpe, Beall, Fagan, Minnich & McKee
Liquid chromatograph and liquid chromatography
 A liquid chromatograph and chromatography which can perform analysis at a higher speed with clearer separation between peaks. The liquid chromatograph comprises a separation column (10), a buffer pump (7) for feeding a plurality of buffers 1, 2, 3 and 4 into the separation column (10), a sampler (9) disposed in a flow path between the buffer pump (7) and the separation column (10) for introducing a sample to be analyzed into the flow path, and a reaction coil (14) for mixing and then reacting amino acids separated by the separation column (10) with a ninhydrin reagent. The separation column is sized so that a ratio (L1/R) of its length L1 to its inner diameter R is not more than 10. The particle size of an ion exchange resin filled in the separation column (10) is selected to be not more than 4 .mu.m. 
Hitachi, Ltd.
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G01N G01N G01N G01N
inventional additional additional additional
Hiroshi Yoshio Masahito
Satake Fujii Ito