IPro, PLLC
Method for reconstructing aspergillus niger to increase citrate production
The invention discloses a method for increasing citrate production from genome reconstructed Aspergillus niger. The method is to insert a gene of low affinity glucose transporter, LGT1, to genome of A. niger. The expression level of LGT1 is under control of promoter Pgas. The genome reconstructed A. niger is tolerant to higher fermentation temperature and lower pH than that of the parental strain. Moreover, the production, yield and purity of product from reconstructed A. niger are higher than that of parental strain, and the fermentation time is shorter.
Jiangnan University
C C C C
C07K C12N C12P C12P
inventional inventional inventional inventional
Sai Zhijie Xian Xiaodong Long Jian Cheng Fuxin Guocheng Jianwei Jianghua
Jin Hu Yin Jiang Liu Chen Zhang Sun Du Jiang Li