Squire Patton Boggs (US) LLP
UDP-dependent glycosyltransferase for high efficiency production of rebaudiosides
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
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Gale Aditi Tina Lishan Wenzong Darren Chantal Michael Shaina
Wichmann Khankhoje Mahatdejkul-Meadows Zhao Li Platt Garcia De Gonzalo Leavell Jackson