Andrews Kurth LLP
Vector for inhibition-based high-throughput screen strategy
A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.
Scinopharm Taiwan, Ltd.
C C C C C
C12N C07K C12N C12N C40B
inventional additional additional inventional inventional
Wei-Kuang Min-Pey
Liu Ding