Bozicevic, Field & Francis LLP
Protein substance having triple helix structure and manufacturing method therefor
As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
Fuso Pharmaceutical Industries, Ltd. National University Corporation Hokkaido University
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Yusuke Yuzuru Keiichi Yasuhiko Hiroshi
Suzuki Kitahara Yamamoto Suzuki Tahara