Howard & Howard Attorneys PLLC
Fluorescent molecular probes for use in assays that measure test compound competitive binding with SAM-utilizing proteins
Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM -utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.
THE REGENTS OF THE UNIVERSITY OF MICHIGAN CAYMAN CHEMICAL COMPANY, INCORPORATED
C G G C G C C G
C07D G01N G01N C07D G01N C07H C07D G01N
inventional additional additional inventional inventional inventional inventional inventional
Ranjinder Singh Jeffrey Keith Margaret Lynn Gregory Scott Stephen Douglas Fred Lawrence Daniel Austin Levi Lynn Raymond C. Gregory William
Sidhu Johnson Collins Keyes Barrett Ciske Bochar Blazer Trievel Endres