Banner & Witcoff, Ltd.
Culture method for amplifying large numbers of hair follicle stem cells in vitro
The present invention is a method for amplifying a large numbers of hair follicle stem cells in vitro by using microspheres as carriers for cell culture and a revolving bottle as a fermentation tank for cell proliferation. The method includes digesting hair follicles to obtain hair follicle stem cells, seeding the cells on microcarriers, transferring the microcarriers to a revolving bottle, feeding liquid culture media into the revolving bottle, placing the revolving bottle into a cell incubator, growing the cells on the microcarrier and harvesting the hair follicle stem cells.
Jilin University
C C
C12N C12N
inventional additional
Yulin Jinyu
Li Liu