Darby & Darby
Process for preparing hepatitis C virus envelope glycoproteins
 The present invention relates to a novel process for preparing hepatitis C virus (HCV) envelope glycoproteins employing Chinese Hamster Ovary (CHO) cells transformed with recombinant expression vectors containing the hepatitis C virus genome. The present invention provides CHO cells cotransfected with DHFR (dihydrofolate reductase) minigene pDCHIP and recombinant expression vectors containing cDNAs of HCV E1 and E2/NS1 ligated with tissue plasminogen activator signal sequence. HCV E1 and E2/NS1 envelope glycoproteins are produced in a massive manner from the transformed CHO cells adapted in methotrexate. The HCV envelope glycoproteins produced by the present invention can be applied to the development of a diagnostic reagent and a potential preventive HCV vaccine. 
Mogam Biotechnology Research Institute
C C A
C12N C07K A61K
additional inventional additional
Mi-Kyung Yung-Dae Jung-Seob Hong-Mo Joon-Sang
Min Yun Kim Moon Park