Kilpatrick Townsend & Stockton LLP
In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
California Institute of Technology
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C12Y C12N C12P Y02P C12P C12N
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Frances H. Pedro S. Zhan Jared C. Eric M.
Arnold Coelho Wang Lewis Brustad