Mintz Levin Cohn Ferris Glovsky and Popeo, P.C.
Reconstituted apolipoprotein B lipoparticle, a preparation method and uses thereof
The present invention provides a method for preparing a reconstituted apolipoprotein B lipoparticle and the method comprises steps of (a) dissolving an apolipoprotein B and a lipid in a first buffer containing 2 M to 8 M urea and 1 wt % to 15 wt % amphiphilic compounds to form a mixture; and (b) dialyzing the mixture against a second buffer containing 0 M to 2M urea and 0 wt % to 0.5 wt % amphiphilic compounds for 1 to several times for lowering concentrations of the urea and the amphiphilic compounds in the mixture. The present invention further provides an apolipoprotein B lipoparticle and a use for the production of an apolipoprotein B lipoparticle used for delivering a hydrophobic substance.
National Chiao Tung University
A A A A
A61K A61K A61K A61K
inventional inventional inventional inventional
Hsueh-Liang Chia-Ching Gong-Shen Tsai-Mu
Chu Chang Chen Cheng