IPro, PLLC
Method for regulating expression of protein of interest in bacillus subtilis
The present disclosure relates to a method for regulating expression of protein of interest in Bacillus subtilis, and belongs to the technical field of genetic engineering. The method comprises: using Bacillus subtilis as an expression host, adding the N-terminal nucleotide sequence coding the first 15 amino acids of the endogenous protein before the coding gene of the protein of interest or modifying the original N-terminal nucleotide sequence coding the first 15 amino acids, and performing free expression in plasmid, thereby regulating expression of the protein of interest in Bacillus subtilis, and even regulating the expression difference in different growth phases and the expression level.
JIANGNAN UNIVERSITY
C C C
C07K C12N C07K
inventional inventional inventional
Rongzhen Guocheng Yanfeng Jian
Tian Du Liu Chen