Johnson, Marcou, Issacs & Nix, LLC
Devices for CRISPR effector system based diagnostics
The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
Massachusetts Institute of Technology President and Fellows of Harvard College The Broad Institute, Inc.
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Omar Feng James Joseph Eric S. Jonathan
Abudayyeh Zhang Collins Lander Gootenberg