Bacon & Thomas
Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine
 The invention relates to a method for the extraction of recombinant periplasmic proteins wherein arginine is used as the extraction agent. In particular, the invention relates to a method for the extraction of a periplasmic protein of interest, which essentially consists in: PA1 (i) suspending the pellet of cells or of cell debris from cells, which cells originate from the culture of a prokaryotic microorganism transformed with an expression vector containing a gene coding for the protein and means for its expression in the periplasm of the microorganism, in a buffer solution containing arginine; and PA1 (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained. 
Sanofi
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Richard Marc Paul
Legoux Salome Maldonado