New England Biolabs, Inc
High fidelity restriction endonucleases
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
G C C G C C C C
G01N C12N C12N G01N C12Q C12Q C12Y C12Q
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Shuang-Yong Xianghui Dapeng Chunhua Aine Zhenyu Xuhui Siu-hong Shengxi Yishu
Xu Li Sun Zhang Quimby Zhu Lai Chan Guan Huang