Method for positioning and integrating transgene and use thereof
Provided in the present invention is a method for positioning and integrating transgene and a use thereof. Specifically provided is a variant loxP element. The sequence “ATAAT” of an reverse repeated sequence in a wild type loxp locus is mutated into “CACCT”, i.e., a variant loxp element. Also provided in the present invention are a construct comprising the variant loxP element, a vector or host cell comprising the construct and a method for preparing a transgenic animal using the vector and the host cell.
SHANGHAI GENON BIOENGINEERING CO., LTD SHANGHAI TRANSGENIC RESEARCH CENTER
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Jianquan Guoxiang Siguo
Chen Cheng Liu