Womble Carlyle Sandridge & Rice LLP
Highly glyphosate-resistant mutated gene, method of modification and use thereof
Disclosed are a method for obtaining highly glyphosate-resistant G1174 gene mutants through artificial mutation and use thereof. Two or more amino acid mutations are introduced in the amino acid sequence from position 95 to position 114 of protein encoded by G1174 gene, and the highly glyphosate-resistant mutants are selected. Also disclosed is a highly glyphosate-resistant mutated G1174 gene, wherein the amino acid sequence from position 95 to position 114 is selected from any one of SEQ ID NO: 4 to SEQ ID NO: 24. The use of the highly glyphosate-resistant mutated gene is for conferring a plant with glyphosate resistance through expression of the transgene in the plant.
HANGZHOU RUIFENG BIOTECHNOLOGY LIMITED INC.
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C12N C07K C12N C12N C12Y
inventional inventional additional inventional additional
Xiaoli Xinlan Zhicheng Chaoyang
Xu Li Shen Lin