Sughrue Mion, PLLC
Measuring thrombin activity in whole blood
The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: —contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; —allowing thrombin to generate in said sample; —measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
Synapse B.V.
G C
G01N C12Q
additional inventional
Hendrik Coenraad Raed Sebastiaan Peter Suzette Robert
Hemker Al-Dieri Nijhuis Giesen Beguin Wagenvoord