Rothwell, Figg, Ernst & Manbeck, P.C.
Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells
The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
Chabio & Diostech Co., Ltd. College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation
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Sung-Hwan Hyung-Min Soo-Hong Ju-Mi
Moon Chung Lee Kim